Other
Part:BBa_K4388012:Design
Designed by: Tomas Lopes Group: iGEM22_KCL_UK (2022-10-08)
Linker Sequence Replacing Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ensured no RFC10, RFC1000, nor JUMP standard restriction enzyme sites were present.
Source
Sequence created as a linker for use in a synthetic operon system, with no BsaI nor BsmBI restriction sites thus allowing for JUMP assembly.